The lab’s current research focuses on how neonatal intraventricular hemorrhage (bleeding into the brain’s ventricles in premature infants) causes inflammation and impedes normal brain development. The lab studies how surgical and new medical interventions can protect the brain from injury.

John Bufalino 10/16/26 John Bufalino 10/16/26

Primary and immortalized microglia exhibit divergent responses to hemoglobin

John Bufalino 3/6/26 John Bufalino 3/6/26

Acute brain inflammation, white matter oxidative stress, and myelin deficiency in a model of neonatal intraventricular hemorrhage

John Bufalino 3/6/26 John Bufalino 3/6/26

Safety of Indocyanine Green Microdosing for Clinical Imaging of CSF Ventricular Dynamics and Extracranial Outflow

John Bufalino 3/6/26 John Bufalino 3/6/26

A Sublethal Dose of TNF Alpha Potentiates Kainate-Induced Excitotoxicity in Optic Nerve Oligodendrocytes

John Bufalino 3/6/26 John Bufalino 3/6/26

Neonatal hydrocephalus leads to white matter neuroinflammation and injury in the corpus callosum of Ccdc39 hydrocephalic mice

John Bufalino 3/6/26 John Bufalino 3/6/26

A lumbar arteriovenous fistula presenting with intraventricular hemorrhage and hydrocephalus

John Bufalino 3/6/26 John Bufalino 3/6/26

Modeling brain injury: neonatal intraventricular hemorrhage

John Bufalino 3/6/26 John Bufalino 3/6/26

Cerebral protection by hypoxic preconditioning in a murine model of focal ischemia-reperfusion

John Bufalino 3/6/26 John Bufalino 3/6/26

Applications of a robotic stereotactic arm for pediatric epilepsy and neurooncology surgery

John Bufalino 2/20/26 John Bufalino 2/20/26

Azithromycin reduces hemoglobin-induced innate neuroimmune activation

John Bufalino 2/20/26 John Bufalino 2/20/26

Innate immune activation and white matter injury in a rat model of neonatal intraventricular hemorrhage are dependent ondevelopmental stage

John Bufalino 2/20/26 John Bufalino 2/20/26

Developmental stage of oligodendrocytes determines their response to activated microglia in vitro

John Bufalino 10/4/22 John Bufalino 10/4/22

Modeling Neonatal Intraventricular Hemorrhage through Intraventricular Injection of Hemoglobin

John Bufalino 9/29/22 John Bufalino 9/29/22

Developmental stage-dependent transcriptomic responses to neonatal intraventricular hemorrhage

Thank you to our past and present supporters who have propelled our research forward.

Current and Recent Awards

NIH / NINDS- R01 NS134626

NIH / NINDS- K08 NS112580

Hydrocephalus Association - Innovator Award

Mission Connect / TIRR Founders Award

Hydrocephalus Association - Accelerator Award

ACADEMIC RIGOR

Dr. Miller brings unique expertise, combining his neurosurgical experience with his neuroscience background. He has been an invited speaker at many professional society conferences and serves as a valued peer reviewer for clinical and scientific journals.